Aldos-2-ulose dehydratase (, pyranosone dehydratase, AUDH, 1,5-anhydro-D-fructose dehydratase (microthecin-forming)) is an enzyme with systematic name 1,5-anhydro-D-fructose hydro-lyase (microthecin-forming). This enzyme catalyses the following chemical reaction
1,5-anhydro-D-fructose 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O (overall reaction) (1a) 1,5-anhydro-D-fructose 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O (1b) 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one
This enzyme catalyses two of the steps in the anhydrofructose pathway.
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