2'-N-acetylparomamine deacetylase (, btrD (gene), neoL (gene), kanN (gene)) is an enzyme characterisd from Bacillus circulans with systematic name 2'-N-acetylparomamine hydrolase (acetate-forming). It catalyses the hydrolysi of 2'-N-acetylparomamine to paromamine and acetic acid:
This enzyme takes part in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin.
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