2'''-acetyl-6'''-hydroxyneomycin C deacetylase (, neoL (gene)) is an enzyme with systematic name 2'''-acetyl-6'''-hydroxyneomycin C hydrolase (acetate-forming). It catalyses the hydrolysis of 2"'-acetyl-6"'-hydroxyneomycin C to 6"'-hydroxyneomycin C and acetic acid:
This enzyme is involved in biosynthesis of aminoglycoside antibiotics of the neomycin family.
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