Ophiolysin (, Ophiophagus metalloendopeptidase) is an enzyme. This enzyme catalyses the following chemical reaction

Cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu, and Tyr16-Leu in insulin B chain

This endopeptidase is present in the venom of the king cobra (Ophiophagus hannah)

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