Rhamnogalacturonan acetylesterase (EC 3.1.1.86, RGAE; systematic name rhamnogalacturonan 2/3-O-acetyl-α-D-galacturonate O-acetylhydrolase) is an enzyme catalyses the reaction

Hydrolytic cleavage of 2-O-acetyl- or 3-O-acetyl groups of α-D-galacturonic acid in rhamnogalacturonan I.

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