23S rRNA (cytosine1962-C5)-methyltransferase (, RlmI, rRNA large subunit methyltransferase I, YccW) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (cytosine1962-C5)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + cytosine1962 in 23S rRNA S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA

The enzyme specifically methylates cytosine1962 at C5 in 23S rRNA.

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