N8-acetylspermidine oxidase (propane-1,3-diamine-forming) () is an enzyme with systematic name N8-acetylspermidine:oxygen oxidoreductase (propane-1,3-diamine-forming). This enzyme catalyses the following chemical reaction
N8-acetylspermidine + O2 + H2O propane-1,3-diamine + 4-acetamidobutanal + H2O2
This enzyme is also active n N1-acetylspermine, and it has weak activity on N1,N12-diacetylspermine.
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