John Jeffrey (born 1959) is a Scottish rugby union player.

John Jeffrey may also refer to:

John Jeffrey (botanist) (1826–1854), Scottish botanist and plant-hunter John Jeffrey (judge), MP for Sussex, East Grinstead and Clitheroe John Jeffrey (civil servant), Permanent Under-Secretary of State for Scotland

See also John Jeffery (disambiguation) Jeffrey John, priest

John Jeffreys (disambiguation) John Jeffries (disambiguation) John Jaffray (disambiguation)