John Jeffries (1745–1819) was a physician and scientist.
John Jeffries may also refer to:
John Calvin Jeffries (born c. 1948), law professor John Jeffries II (1796–1876), American ophthalmic surgeon John Jeffries (judge) (1929-2019), former judge, local politician and senior civil servant
See also John Jeffries Award, awarded to Harry George Armstrong John Jeffreys (disambiguation) John Jeffrey (disambiguation)