List of restriction enzyme cutting sites: Bsa–Bso

This article contains a list of the most studied restriction enzymes whose names start with Bsa to Bso inclusive. It contains approximately 90 enzymes. The following information is given:

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Restriction enzymes

Bsa - Bso

Enzyme PDB code Source Recognition sequence Cut IsoschizomersBsaI Bacillus stearothermophilus 20241 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' Bso31I, BspTNI, Eco31IBsa29I Bacillus sp. 29 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, BavCI, BseCI, BspDI, Bsu15I, BsuTUI, ClaI, BbvAIIBsaAI Bacillus stearothermophilus G668 5' YACGTR 3' RTGCAY 5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5' BsaBI Bacillus stearothermophilus B674 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' BsaHI Bacillus stearothermophilus CPW11 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, BbiII, BstACI, HgiI, PamIIBsaJI Bacillus stearothermophilus J695 5' CCNNGG 3' GGNNCC 5' ---C CNNGG--- 3' 3' ---GGNNC C--- 5' BsaMI Bacillus stearothermophilus M293 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp35HI, Asp36HI, Asp50HI, BsaMI, BsmI, Mva1269IBsaOI Bacillus stearothermophilus O-122 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5' BsaWI Bacillus stearothermophilus W1718 5' WCCGGW 3' WGGCCW 5' ---W CCGGW--- 3' 3' ---WGGCC W--- 5' BsaXI Bacillus stearothermophilus 25B 5' ACN5CTCC 3' TGN5GAGG 5' ---ACN5CTCCN6NNNN --- 3' 3' ---TGN5GAGGN6N NNN--- 5' BsmCI, BsmDI, BsmXIBscI Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BavCI, Bci29I, BdiI, Bli41I, BliAI, LplI, PgaI, SpmIBsc4I Bacillus schlegelii 4 5' CCN7GG 3' GGN7CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5' Bsc91I Bacillus sp. 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' Bsc107I Bacillus schlegelii 107 5' CCN7GG 3' GGN7CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5' BscAI Bacillus schlegelii S3 5' GCATC 3' CGTAG 5' ---GCATCNNNN NN--- 3' 3' ---CGTAGNNNNNN --- 5' BscBI Bacillus sp. A11 5' GGNNCC 3' CCNNGG 5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5' AspNI, BmiI, BspLI, NlaIV, PspN4IBscCI Bacillus sp. 2G 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, PctIBscFI Bacillus sp. JY391 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BtkII, CviAI, Kzo9I, NlaII, SsiBIBse1I Bacillus stearothermophilus 1 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5' Bse8I Bacillus sp. 8 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' Bse15I Bacillus sp. 15 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' Ama87I, AvaI, BcoI, BsoBI, BstSI, Eco88I, NspSAI, OfoIBse16I Bacillus sp. 16 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, BsiLI, BstNI, BstM6I, BthDI, BthEI, EcoRII, MvaIBse17I Bacillus sp. 17 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGIBse21I Bacillus sp. 21 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAIBse24I Bacillus sp. 24 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGIBse64I Bacillus sp. 64 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AspAI, Bse64I, BseT9I, BstPI, EcaI, Eci125I, EcoO65I, NspSAIIBse118I Bacillus sp. 118 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' Bse634I 1KNV Bacillus sp. 634 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' BseAI Bacillus sphaericus 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, Aor13HI, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, Kpn2IBseBI Bacillus stearothermophilus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, AorI, Bse17I, Bst1I, BstOI, Bst2UI, EcoRII, MvaI, SleI, ZanIBseCI Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, BavCI, Bsa29I, BspDI, Bsu15I, BsuTUI, ClaI, LcaIBseDI Bacillus stearothermophilus RFL1434 5' CCNNGG 3' GGNNCC 5' ---C CNNGG--- 3' 3' ---GGNNC C--- 5' Bse3DI Bacillus stearothermophilus 3D 5' GCAATG 3' CGTTAC 5' ---GCAATGNN --- 3' 3' ---CGTTAC NN--- 5' BseGI Bacillus stearothermophilus Vs 34-031 5' GGATG 3' CCTAC 5' ---GGATGNN --- 3' 3' ---CCTAC NN--- 5' BseJI Bacillus stearothermophilus Tsp5 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' BseKI Bacillus stearothermophilus Ra3-212 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BbvI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1IBseLI Bacillus stearothermophilus LK3-551 5' CCN7GG 3' GGN7CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5' BseMI Bacillus stearothermophilus Isl 15-111 5' GCAATG 3' CGTTAC 5' ---GCAATGNN --- 3' 3' ---CGTTAC NN--- 5' BseMII Bacillus stearothermophilus Isl 15-111 5' CTCAG 3' GAGTC 5' ---CTCAGN7NNN --- 3' 3' ---GAGTCN7N NN--- 5' BseNI Bacillus sp. N 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5' BsePI Bacillus stearothermophilus P6 5' GCGCGC 3' CGCGCG 5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5' BseQI Bacillus sp. Q 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BseRI Bacillus sp. R 5' GAGGAG 3' CTCCTC 5' ---GAGGAGN7NNN --- 3' 3' ---CTCCTCN7N NN--- 5' BseSI Bacillus stearothermophilus Jo-553 5' GKGCMC 3' CMCGKG 5' ---GKGCM C--- 3' 3' ---C MCGKG--- 5' BseT9I Bacillus sp. T9 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, Bse64I, BstEII, BstT9I, EcaI, Eci125I, EcoO65I, NspSAII, PspEIBseT10I Bacillus sp. T10 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstT10I, BstPI, EcoO128I, EcoO65I, PspEIBseXI Bacillus stearothermophilus Ra3-212 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BbvI, BseKI, Bsp423I, Bst12I, Bst71I, BstV1IBseX3I Bacillus stearothermophilus X3 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIIIBseYI Bacillus sp. 2521 5' CCCAGC 3' GGGTCG 5' ---C CCAGC--- 3' 3' ---GGGTC G--- 5' BseZI Bacillus sp. Z 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' BsgI Bacillus sphaericus B922 5' GTGCAG 3' CACGTC 5' ---GTGCAGN12NNNN --- 3' 3' ---CACGTCN12NN NN--- 5' BshI Bacillus sphaericus 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' Bsh45I Bacillus sphaericus 45 5' GWGCWC 3' CWCGWG 5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5' Alw21I, AspHI, Bbv12I, BsiHKAI,

HgiAI, HpyF46II, MspV281IBsh1236I Bacillus sphaericus RFL1236 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, BceBI, BspFNI, Bsp50I, Csp68KVI, FauBII, MvnI, SelIBsh1285I Bacillus sphaericus RFL1285 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5' Bsh1365I Bacillus sphaericus RFL1365 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' BshFI Bacillus sphaericus 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BshGI Bacillus sphaericus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, PspGI, SspAIBshKI Bacillus sphaericus 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, Bsp1894I, BspBII, CcuI, MaeK81II, Pde12IBshNI Bacillus sphaericus TK-45 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' BanI, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAIBshTI Bacillus sphaericus Jo22-024 5' ACCGGT 3' TGGCCA 5' ---A CCGGT--- 3' 3' ---TGGCC A--- 5' AgeI, AsiAI, AsiGI, CsiAI CspAI, PinAIBsiI Bacillus sphaericus 5' CACGAG 3' GTGCTC 5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5' BsiBI Bacillus sp. 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' BsiCI Bacillus sp. 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, AsuII, Bpu14I, BspT104I, Csp45I, FspII, NspV, PpaAI, SfuIBsiEI Bacillus sp. 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5' BsiHKAI Bacillus stearothermophilus 5' GWGCWC 3' CWCGWG 5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5' Alw21I, AspHI, Bbv12I, Bsh45I, Bsm6I, HgiAI, MspV281IBsiHKCI Bacillus sp. HKC 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, Bse15I, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAIBsiKI Bacillus sp. 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' BsiLI Bacillus sp. 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AglI, BseBI, Bse17I, Bst2I, BstNI, Bst38I, Bst100I, EcoRII, MvaIBsiMI Bacillus sp. 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, Bsp13I, BspMII, Bsu23I, Kpn2I, PinBIIBsiQI Bacillus sp. 5' TGATCA 3' ACTAGT 5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' AbaI, BclI, BspXII, BstT7I, FbaI, Ksp22I, ParIBsiSI Bacillus sp. 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' BsiWI Bacillus sp. 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5' BsiXI Bacillus sp. X 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BscI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, Rme21IBsiYI Bacillus sp. 5' CCN7GG 3' GGN7CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5' BsiZI Bacillus sp. 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, MaeK81II, Pde12IBslI Bacillus sp. 5' CCN7GG 3' GGN7CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5' BslFI Bacillus stearothermophilus FI 5' GGGAC 3' CCCTG 5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' BsmI Bacillus stearothermophilus NUB 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp36HI, Asp40HI, BmaHI, BscCI, Mva1269I, PctIBsmAI Bacillus stearothermophilus A664 5' GTCTC 3' CAGAG 5' ---GTCTCN NNNN--- 3' 3' ---CAGAGNNNNN --- 5' Alw26I, BcoDI, BscQII, BsoMAI, BstMAIBsmBI Bacillus stearothermophilus B61 5' CGTCTC 3' GCAGAG 5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5' BsmFI Bacillus stearothermophilus F 5' GGGAC 3' CCCTG 5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' BsmSI Bacillus stearothermophilus CP114 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' Bso31I Bacillus stearothermophilus E31 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' BsoBI 1DC1 Bacillus stearothermophilus JN2091 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAIBsoCI Bacillus stearothermophilus AU891 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BmyI, Bsp1286I, BspLS2I, MhlI, NspII, SduIBsoFI Bacillus stearothermophilus F66 5' GCNGC 3' CGNCG 5' ---GC NGC--- 3' 3' ---CGN CG--- 5' BsoMAI Bacillus stearothermophilus MA 5' GTCTC 3' CAGAG 5' ---GTCTCN NNNN--- 3' 3' ---CAGAGNNNNN --- 5' Alw26I, BcoDI, BscQII, BsmAI, BstMAI

Notes