This article contains a list of the most studied restriction enzymes whose names start with C to D inclusive. It contains approximately 80 enzymes.
The following information is given:
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Restriction enzymes
C
Enzyme PDB code Source Recognition sequence Cut IsoschizomersCacI Clostridium acetobutylicum N1-4081 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Cac8I Clostridium acetobutylicum ABKn8 5' GCNNGC 3' CGNNCG 5' ---GCN NGC--- 3' 3' ---CGN NCG--- 5' CaiI Comamonas acidovarans Iti19-021 5' CAGNNNCTG 3' GTCNNNGAC 5' ---CAGNNN CTG--- 3' 3' ---GTC NNNGAC--- 5' AlwNICauI Chloroflexus aurantiacus 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiCII, HgiJI, SmuEICauII Chloroflexus aurantiacus 5' CCSGG 3' GGSCC 5' ---CC SGG--- 3' 3' ---GGS CC--- 5' AhaI, AseII, AsuC2I, BcnI, Eco1831I, EcoHI, Kpn49kII, NciICauB3I Chloroflexus aurantiacus B3 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' Aor13HI, BbvAIII, BseAI, BspEI, BspMII, Kpn2I, MroI, PtaICbiI Clostridium bifermentans B-4 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, Bim19I, BsiCI, BspT104I, Csp45I, LspI, NspV, SspRFICboI Clostridium botulinum 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' CbrI Citrobacter braakii 1146 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaICciNI Curtobacterium citreus 5' GCGGCCGC 3' CGCCGGCG 5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5' CcoI Clostridium coccoides B-2 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' CcrI Caulobacter crescentus CB-13 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' CcuI Chroococcidiopsis cubana 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bal228I, BavBII, BsiZI, BspF4I, Cfr13I, Nsp7121I, PspPICcyI Clostridium cylindrosporum 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BfuCI, Bsp67I, Bst19II, CviAI, FnuCI, MboI, NmeCI, SauMICdiI Citrobacter freundii RFL2 5' CATCG 3' GTAGC 5' ---CATC G--- 3' 3' ---GTAG C--- 5' CelI Citrobacter freundii RFL6 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AliI, ApaCI, AsiI, BamHI, CelI, NspSAIV, RspLKII, SolI, Uba4009ICelII Coccochloris elabens 17a 5' GCTNAGC 3' CGANTCG 5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5' CeqI Corynebacterium equi 5' GATATC 3' CTATAG 5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5' CflI Cellulomonas flavigena 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, BloHII, CflI, CstI, Ecl37kI, PaePI, PstI, SalPI, Srl5DI, Sst12ICfoI Clostridium formicoaceticum 5' GCGC 3' CGCG 5' ---GCG C--- 3' 3' ---C GCG--- 5' AspLEI, BspLAI, BstHHI, FnuDIII, HhaI, HinP1I, HsoI, HspAI, SciNICfrI Citrobacter freundii RFL2 5' YGGCCR 3' RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' Cfr6I Citrobacter freundii RFL6 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' Cfr9I Citrobacter freundii 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCICfr10I1CFR Citrobacter freundii RFL10 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' Cfr13I Citrobacter freundii RFL13 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bal228I, BavBII, BsiZI, BspF4I, FmuI, Nsp7121I, PspPICfr42I Citrobacter freundii RFL42 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' SacII, CscI, HgaICfrA4I Citrobacter freundii A4 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, BloHII, CflI, CstI, Ecl2zI, PaePI, PstI, SalPI, Srl5DI, Sst12ICfrBI Citrobacter freundii 4111 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' CfrJ4I Citrobacter freundii J4 5' CCCGGG 3' GGGCCC 5' ---CCC GGG--- 3' 3' ---GGG CCC--- 5' AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, SmaI, TspMI, XcyI, XmaICfuI Caulobacter fusiformis BC-25 5' GATC 3' CTAG 5' ---GA TC--- 3' 3' ---CT AG--- 5' CfuII Caulobacter fusiformis BC-25 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, AliAJI, CfuII, HalII, PaePI, Psp23I, PstI, SalPI, Srl5DI, YenIChaI Corynebacterium halofytica 5' GATC 3' CTAG 5' ---GATC --- 3' 3' --- CTAG--- 5' BfuCI, Bsp2095I, BspKT6I BtkII, FnuCI, MboI, NmeCI, SsiAICjeI Campylobacter jejuni P37 5' CCAN6GT 3' GGTN6CA 5' ---CCAN6GTN8NNNNNNN --- 3' 3' ---GGTN6CAN8N NNNNNN--- 5' — None in May 2010 —CjePI Campylobacter jejuni P116 5' CCAN7TC 3' GGTN7AG 5' ---CCAN7TCN7NNNNNNN --- 3' 3' ---GGTN7AGN7N NNNNNN--- 5' — None in May 2010 —ClaI Caryophanon latum L 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUICltI Caryophanon latum 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' CpfI Clostridium perfringens 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' AspMDI, Bsp67I, Bst19II, CcyI, FnuCI, MboI, NphI, SauMICpoI Caseobacter polymorphus 5' CGGWCCG 3' GCCWGGC 5' ---CG GWCCG--- 3' 3' ---GCCWG GC--- 5' CscI Calothrix scopulorum 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Cfr42I, SacII, HgaICsiAI Corynebacterium sp. A 5' ACCGGT 3' TGGCCA 5' ---A CCGGT--- 3' 3' ---TGGCC A--- 5' AgeI, AsiAI, AsiGI, BshTI CspAI, PinAICsiBI Corynebacterium sp. B 5' GCGGCCGC 3' CGCCGGCG 5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5' CspI Corynebacterium sp. 5' CGGWCCG 3' GCCWGGC 5' ---CG GWCCG--- 3' 3' ---GCCWG GC--- 5' Csp6I Corynebacterium sp. RFL6 5' GTAC 3' CATG 5' ---G TAC--- 3' 3' ---CAT G--- 5' AfaI, CviQI, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI,Csp45I Clostridium sporogenes 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, Bim19I, BsiCI, BspT104I, FspII, LspI, NspV, SspRFICspAI Corynebacterium sp. 301 5' ACCGGT 3' TGGCCA 5' ---A CCGGT--- 3' 3' ---TGGCC A--- 5' AgeI, AsiAI, AsiGI, BshTI CsiAI, PinAICspBI Corynebacterium sp. B 5' GCGGCCGC 3' CGCCGGCG 5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5' CspCI Citrobacter sp. 2144 5' CAAN4NGTGG 3' GTTN4NCACC 5' ---CAAN5GTGGN8NNNN --- 3' 3' ---GTTN5CACCN8NN NN--- 5' — None in May 2010 —Csp68KI Cyanothece sp. BH68K 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiCII, Kzo49I, SmuEICsp68KII Cyanothece sp. BH68K 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, Bim19I, BsiCI, BstBI, Csp45I, LspI, PlaII, SviICsp68KIII Cyanothece sp. BH68K 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' BfrBI, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2ICsp68KVI Cyanothece sp. BH68K 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' BepI, Bpu95I, Bsp123I, BstFNI, BstUI, BtkI, FalII, SelI, ThaICspKVI Cyanothece sp. BH68K 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' CstI Clostridium sticklandii 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, AliAJI, CfrA4I, HalII, MhaAI, Pfl21I, PstI, SflI, Srl5DI, YenICstMI Corynebacterium striatum M82B 5' AAGGAG 3' TTCCTC 5' ---AAGGAGN17NNN --- 3' 3' ---TTCCTCN17N NN--- 5' — None in May 2010 —CthII Clostridium thermocellum 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' ApaORI, BseBI, Bse24I, Bst2UI, BstNI, BstM6I, EcoRII, MvaICviAI Chlorella NC64A (PBCV-1) 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bme12I, Bsp67I, Bst19II, CcyI, FnuCI, MgoI, NphI, SauMICviAII Chlorella NC64A (PBCV-1) 5' CATG 3' GTAC 5' ---C ATG--- 3' 3' ---GTA C--- 5' CviBI Chlorella NC64A (NC-1A) 5' GANTC 3' CTNAG 5' ---G ANTC--- 3' 3' ---CTNA G--- 5' CviJI Chlorella NC64A (IL-3A) 5' RGCY 3' YCGR 5' ---RG CY--- 3' 3' ---YC GR--- 5' CviQI Chlorella NC64A (NY-2A) 5' GTAC 3' CATG 5' ---G TAC--- 3' 3' ---CAT G--- 5' AfaI, Csp6I, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI,CviRI Chlorella NC64A (XZ-6E) 5' TGCA 3' ACGT 5' ---TG CA--- 3' 3' ---AC GT--- 5' CviRII Chlorella NC64A (XZ-6E) 5' GTAC 3' CATG 5' ---G TAC--- 3' 3' ---CAT G--- 5' AfaI, Csp6I, CviQI, HpyBI, PabI, PlaAII, RsaI, RsaNI,CviTI Chlorella NC64A (CA-1A) 5' RGCY 3' YCGR 5' ---RG CY--- 3' 3' ---YC GR--- 5' CvnI Chromatium vinosum 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAI
D
Enzyme PDB code Source Recognition sequence Cut IsoschizomersDdeI Desulfovibrio desulfuricans 5' CTNAG 3' GANTC 5' ---C TNAG--- 3' 3' ---GANT C--- 5' BstDEI, HpyF3I DmaI Deleya marina 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' DpaI Deleya pacifica 5' AGTACT 3' TCATGA 5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5' Acc113I, AssI, BmcAI, Bpa34I, Eco255I, RflFII, ScaI, ZrmIDpnI Diplococcus pneumoniae G41 5' GATC 3' CTAG 5' ---GA TC--- 3' 3' ---CT AG--- 5' DpnII Diplococcus pneumoniae G41 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' AspMDI, Bsp105I, BspFI, BstMBI, CpfI, Kzo9I, NdeII, Sth368IDraI Deinococcus radiophilus 5' TTTAAA 3' AAATTT 5' ---TTT AAA--- 3' 3' ---AAA TTT--- 5' AhaIII, PauAII, SruIDraII Deinococcus radiophilus 5' RGGNCCY 3' YCCNGGR 5' ---RG GNCCY--- 3' 3' ---YCCNG GR--- 5' DraIII Deinococcus radiophilus 5' CACNNNGTG 3' GTGNNNCAC 5' ---CACNNN GTG--- 3' 3' ---GTG NNNCAC--- 5' AdeI, BstIZ316IDrdI Deinococcus radiodurans 5' GACN6GTC 3' CTGN6CAG 5' ---GACNNNN NNGTC--- 3' 3' ---CTGNN NNNNCAG--- 5' AasI, DseDIDriI Dactylococcopsis salina 5' GACN5GTC 3' CTGN5CAG 5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5' AhdI, AspEI, BmeRI, BspOVI, Eam1105I, EclHKI, NruGIDsaI Dactylococcopsis salina 5' CCRYGG 3' GGYRCC 5' ---C CRYGG--- 3' 3' ---GGYRC C--- 5' DsaII Dactylococcopsis salina 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' DsaIII Dactylococcopsis salina 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' DsaIV Dactylococcopsis salina 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiEI, Kzo49I, SmuEIDsaV Dactylococcopsis salina 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' DseDI Deinococcus sp. Dx 5' GACN6GTC 3' CTGN6CAG 5' ---GACNNNN NNGTC--- 3' 3' ---CTGNN NNNNCAG--- 5' AasI, DrdI
Notes