This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given:

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Restriction enzymes

S

Enzyme PDB code Source Recognition sequence Cut IsoschizomersSacI Streptomyces achromogenes 5' GAGCTC 3' CTCGAG 5' ---GAGCT C--- 3' 3' ---C TCGAG--- 5' SacII Streptomyces achromogenes 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Cfr42ISacNI Streptomyces achromogenes N-J-H 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' SalI Streptomyces albus 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' SalPI Streptomyces albus 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenISanDI Streptomyces sp. 5' GGGWCCC 3' CCCWGGG 5' ---GG GWCCC--- 3' 3' ---CCCWG GG--- 5' SapI Saccharopolyspora sp. 5' GCTCTTC 3' CGAGAAG 5' ---GCTCTTCN NNN--- 3' 3' ---CGAGAAGNNNN --- 5' SarI Streptomyces aureomonopodiales 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuISatI Staphylococcus arlettae RFL832 5' GCNGC 3' CGNCG 5' ---GC NGC--- 3' 3' ---CGN CG--- 5' SauI Streptomyces aureofaciens IKA 18/4 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAISau96I Staphylococcus aureus PS96 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbISau3239I Streptomyces aureofaciens 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaISau3AI 2REU Staphylococcus aureus 3A 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368ISauBMKI Streptomyces aureofaciens BM-K 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' SauHPI Streptomyces aureofaciens R8/26 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' SauLPI Streptomyces aureofaciens B-96 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' SauLPII Streptomyces aureofaciens B-96 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaISauMI Staphylococcus aureus M 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAISauNI Streptomyces aureofaciens NMU 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' SauSI Streptomyces aureofaciens 16 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' SbfI Streptomyces sp. BF-61 5' CCTGCAGG 3' GGACGTCC 5' ---CCTGCA GG--- 3' 3' ---GG ACGTCC--- 5' Sbi68I Streptomyces bikiniensis JAM68 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaISbo13I Shigella boydii 13 5' TCGCGA 3' AGCGCT 5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5' SbvI Streptococcus bovis II/1 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' ScaI Streptomyces caespitosus 5' AGTACT 3' TCATGA 5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5' Acc113I, AssI, BmcAI, Bpa34I, DpaI, Eco255I, RflFII, ZrmISceIII Saccharomyces cerevisiae 5' GCCGGC 3' CGGCCG 5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5' SchI Staphylococcus cohnii Lki 19-320 5' GAGTC 3' CTCAG 5' ---GAGTCN4N --- 3' 3' ---CTCAGN4N --- 5' SchZI Streptomyces chusanensis ZS-2 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' SciI Streptoverticillium cinnamoneum 5' CTCGAG 3' GAGCTC 5' ---CTC GAG--- 3' 3' ---GAG CTC--- 5' BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoISciNI Spiroplasma citri ASP2 5' GCGC 3' CGCG 5' ---G CGC--- 3' 3' ---CGC G--- 5' BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAIScrFI Streptococcus cremoris F 5' CCNGG 3' GGNCC 5' ---CC NGG--- 3' 3' ---GGN CC--- 5' SdaI 2IXS Streptomyces diastaticus Ng7-324 5' CCTGCAGG 3' GGACGTCC 5' ---CCTGCA GG--- 3' 3' ---GG ACGTCC--- 5' SdiI Streptomyces diastaticus 5' GGCCN5GGCC 3' CCGGN5CCGG 5' ---GGCCNNNN NGGCC--- 3' 3' ---CCGGN NNNNCCGG--- 5' SduI Streptococcus durans RFL3 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspIISecI Synechocystis sp. 6701 5' CCNNGG 3' GGNNCC 5' ---C CNNGG--- 3' 3' ---GGNNC C--- 5' SelI Synechococcus elongatus 5' CGCG 3' GCGC 5' --- CGCG--- 3' 3' ---GCGC --- 5' AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVISenPT16I Salmonella enteritidis PT16 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIIISenPT14bI Salmonella enteritidis PT14b 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' SepI Staphylococcus epidermidis 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2ISexAI Streptomyces exfoliatus 5' ACCWGGT 3' TGGWCCA 5' ---A CCWGGT--- 3' 3' ---TGGWCC A--- 5' SexBI Streptomyces exfoliatus 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' SexCI Streptomyces exfoliatus 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' SfaI Streptococcus faecalis zymogenes 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' SfaNI Streptococcus faecalis ND547 5' GCATC 3' CGTAG 5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5' SfcI Streptococcus faecium 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' SfeI Streptococcus faecalis 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' SfiI 2F03 Streptomyces fimbriatus 5' GGCCN5GGCC 3' CCGGN5CCGG 5' ---GGCCNNNN NGGCC--- 3' 3' ---CCGGN NNNNCCGG--- 5' SflI Streptoverticillium flavopersicum 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenISfoI Serratia fonticola 5' GGCGCC 3' CCGCGG 5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5' Sfr274I Streptomyces fradiae 274 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliISfr303I Streptomyces fradiae 303 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' SfuI Streptomyces fulvissimus 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviISgfI Streptomyces griseoruber 5' GCGATCGC 3' CGCTAGCG 5' ---GCGAT CGC--- 3' 3' ---CGC TAGCG--- 5' AsiSI,