List of restriction enzyme cutting sites: L–N

This article contains a list of the most studied restriction enzymes whose names start with L to N inclusive. It contains approximately 120 enzymes.

The following information is given:

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Restriction enzymes

L

Enzyme PDB code Source Recognition sequence Cut IsoschizomersLcaI Lactobacillus casei 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BdiI, Bli41I, Bsa29I, BscI, Bsp106I, BspDI, Bst28I, LplI, PgaI, ZhoILlaAI Lactococcus lactis cremoris W9 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bme12I, Bsp67I, BstENII, ChaI, FnuEI, MgoI, NphI, RalF40ILlaBI Lactococcus lactis cremoris W56 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' LlaCI Lactococcus lactis W15 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' LlaG2I Lactococcus lactis cremoris 5' GCTAGC 3' CGATCG 5' ---G CTAGC--- 3' 3' ---CGATC G--- 5' AceII, AsuNHI, BmtI, BspOI, NheI, PstNHILmu60I Listeria murrayi 60 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, AxyI, BspR7I, CvnI, Lmu60I, MstII, SauI, SshAILplI Lactobacillus plantarum 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BbvAII, BdiI, Bsa29I, BscI, BstNZ169I, ClaI, LcaI, PgaILpnI Legionella pneumophila 5' RGCGCY 3' YCGCGR 5' ---RGC GCY--- 3' 3' ---YCG CGR--- 5' AccB2I, BfoI, Bme142I, Bsp143II, BstH2I, HaeIILspI Lactobacillus sp. 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, BimI, BsiCI, BstBI, Csp45I, PlaII, PpaAI, Ssp1ILweI Listeria welshimeri RFL131 5' GCATC 3' CGTAG 5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5'

M

Enzyme PDB code Source Recognition sequence Cut IsoschizomersMabI Microbacterium arborescens SE 5' ACCWGGT 3' TGGWCCA 5' ---A CCWGGT--- 3' 3' ---TGGWCC A--- 5' MaeI Methanococcus aeolicus PL-15/H 5' CTAG 3' GATC 5' ---C TAG--- 3' 3' ---GAT C--- 5' MaeII Methanococcus aeolicus 5' ACGT 3' TGCA 5' ---A CGT--- 3' 3' ---TGC A--- 5' MaeIII Methanococcus aeolicus 5' GTNAC 3' CANTG 5' --- GTNAC--- 3' 3' ---CANTG --- 5' MaeK81I Microcystis aeruginosa K-81 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5' MaeK81II Microcystis aeruginosa 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96IMamI Microbacterium ammoniaphilum 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' MavI Mycobacterium avium 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SciI, SlaI, Sol10179I, XhoIMbiI Moraxella bovis Fr1-022 5' CCGCTC 3' GGCGAG 5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5' AccBSI, BsrBI, BstD102I, Bst31NIMboI Moraxella bovis 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' AspMDI, Bsp105I, BspFI, BstMBI, CviAI, Kzo9I, NdeII, Sth368IMboII Moraxella bovis 5' GAAGA 3' CTTCT 5' ---GAAGAN6NN --- 3' 3' ---CTTCTN6N N--- 5' MchI Micromonospora chalcea 5' GGCGCC 3' CCGCGG 5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5' MchAI Mycobacterium chelonae 5' GCGGCCGC 3' CGCCGGCG 5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5' MchAII Mycobacterium chelonae 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' McrI Micrococcus cryophilus 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5' MfeI Mycoplasma fermentans 5' CAATTG 3' GTTAAC 5' ---C AATTG--- 3' 3' ---GTTAA C--- 5' MflI Microbacterium flavum 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' MfoAI Mycobacterium fortuitum TMC 1529 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' Mgl14481I Merismopedia glauca 1448-1 5' CCSGG 3' GGSCC 5' ---CC SGG--- 3' 3' ---GGS CC--- 5' AseII, AsuC2I, BcnI, BpuMI, CauII, Eco1831I, EcoHI, HgiS22I, NciIMgoI Mycobacterium gordonae 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, NphI, Sau3AIMhaAI Mycobacterium habana 206 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AliAJI, Asp713I, Bsp63I, CstI, Ecl37kI, HalII, PaePI, PstI, YenIMhlI Micrococcus halobius SD 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, SduIMkrAI Micrococcus kristinae 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bme12I, BspAI, BstENII, ChaI, FnuEI, MgoI, NphI, Sau3AIMlaI Mastigocladus laminosus 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, BimI, BsiCI, BstBI, Csp68KII, LspI, PlaII, Ssp1IMlaAI Micromonospora lacustris 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, BstVI, PanI, SauLPII, Sbi68I, Sfr274I, SlaI, XhoIMlsI Micrococcus luteus Ng 16-122 5' TGGCCA 3' ACCGGT 5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5' MltI Micrococcus luteus 5' AGCT 3' TCGA 5' ---AG CT--- 3' 3' ---TC GA--- 5' AluBI, AluIMluI Micrococcus luteus 5' ACGCGT 3' TGCGCA 5' ---A CGCGT--- 3' 3' ---TGCGC A--- 5' Mlu23I Micrococcus luteus 23 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' Mlu31I Micrococcus luteus 31 5' TGGCCA 3' ACCGGT 5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5' MluB2I Micrococcus luteus B2 5' TCGCGA 3' AGCGCT 5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5' MluNI Micrococcus luteus N 5' TGGCCA 3' ACCGGT 5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5' MlyI Micrococcus lylae 5' GAGTC 3' CTCAG 5' ---GAGTCN4N --- 3' 3' ---CTCAGN4N --- 5' Mly113I Micrococcus lylae 113 5' GGCGCC 3' CCGCGG 5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5' MmeI Methylophilus methylotrophus 5' TCCRAC 3' AGGYTG 5' ---TCCRACN17NNN --- 3' 3' ---AGGYTGN17N NN--- 5' MnlI Moraxella nonliquefaciens 5' CCTC 3' GGAG 5' ---CCTCN4NNN --- 3' 3' ---GGAGN4NN N--- 5' MnoI Moraxella nonliquefaciens 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' Mph1103I Moraxella phenylpyruvica RFL1103 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5IIMroI Micrococcus roseus 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' BbvAIII, BseAI, Bsp13I, BspEI, Bsu23I, CauB3I, PinBII, PtaIMroNI Micrococcus roseus N 5' GCCGGC 3' CGGCCG 5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5' MroXI Micrococcus roseus XM 5' GAAN4TTC 3' CTTN4AAG 5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, PdmI, XmnIMscI Micrococcus sp. 5' TGGCCA 3' ACCGGT 5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5' MseI Micrococcus sp. 5' TTAA 3' AATT 5' ---T TAA--- 3' 3' ---AAT T--- 5' MslI Moraxella osloensis 5' CAYN4RTG 3' GTRN4YAC 5' ---CAYNN NNRTG--- 3' 3' ---GTRNN NNYAC--- 5' MspI 1SA3, , Search PDBe Moraxella sp. 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' Msp17I Micrococcus sp. 17 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Hin1I, Msp17I, PamIIMsp20I Micrococcus sp. 5' TGGCCA 3' ACCGGT 5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5' Msp67I Micrococcus sp. MS67 5' CCNGG 3' GGNCC 5' ---CC NGG--- 3' 3' ---GGN CC--- 5' MspA1I Moraxella sp. A1 5' CMGCKG 3' GKCGMC 5' ---CMG CKG--- 3' 3' ---GKC GMC--- 5' MspB4I Moraxella sp. B4 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, PfaAIMspCI Micrococcus sp. 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' MspR9I Micrococcus sp. R9 5' CCNGG 3' GGNCC 5' ---CC NGG--- 3' 3' ---GGN CC--- 5' MspSWI Moraxella sp. SW 5' ATTTAAAT 3' TAAATTTA 5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5' MspV281I Myxosarcinia sp. V/281 5' GWGCWC 3' CWCGWG 5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5' Alw21I, AspHI, Bbv12I, Bsh45I, BsiHKAI, Bsm6I, HgiAI, HpyF46II,MspYI Microcystis sp. J2 5' YACGTR 3' RTGCAY 5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5' MssI Methylobacterium sp. Dd 5-732 5' GTTTAAAC 3' CAAATTTG 5' ---GTTT AAAC--- 3' 3' ---CAAA TTTG--- 5' PmeIMstI Microcoleus sp. 5' TGCGCA 3' ACGCGT 5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, FspI, NsbI, PamI, Pun14627IMstII Microcoleus sp. 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAIMthZI Methanobacterium thermoformicicum Z-245 5' CTAG 3' GATC 5' ---C TAG--- 3' 3' ---GAT C--- 5' MunI 1D02 Mycoplasma sp. 5' CAATTG 3' GTTAAC 5' ---C AATTG--- 3' 3' ---GTTAA C--- 5' MvaI , , Search PDBe, 2OA9 Micrococcus varians RFL19 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, Bst2UI, SslI, TaqXI, ZanIMva1269I Micrococcus varians RFL1269 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp40HI, Asp50HI, BscCI, BsmI, Mva1269I, PctIMvnI Methanococcus vannielii 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, Bsh1236I, BstFNI, BtkI, Csp68KVI, FalII, FauBII, ThaIMvrI Micrococcus varians 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa22MI, BspCI, EagBI, ErhB9I, Ple19I, PvuI, RshI, XorIIMwoI Methanobacterium wolfei 5' GCN7GC 3' CGN7CG 5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' MxaI Myxococcus xanthus F18E 5' GAGCTC 3' CTCGAG 5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5'

N

Enzyme PDB code Source Recognition sequence Cut IsoschizomersNaeI 1EV7, , Search PDB Nocardia aerocolonigenes 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' NarI Nocardia argentinensis 5' GGCGCC 3' CCGCGG 5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5' NblI Nocardia blackwellii 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, Afa22MI, EagBI, MvrI, Ple19I, Psu161I, RshI, XorIINciI Neisseria cinerea 5' CCSGG 3' GGSCC 5' ---CC SGG--- 3' 3' ---GGS CC--- 5' AhaI, AseII, AsuC2I, BpuMI,

CauII, EcoHI, HgiS22I, Mgl14481INcoI Nocardia corallina 5' CCATGG 3' GGTACC 5' ---C CATGG--- 3' 3' ---GGTAC C--- 5' NcrI Nocardia carnea C-212 5' AGATCT 3' TCTAGA 5' ---A GATCT--- 3' 3' ---TCTAG A--- 5' NcuI Neisseria cuniculi 5' GAAGA 3' CTTCT 5' ---GAAGAN6NN --- 3' 3' ---CTTCTN6N N--- 5' NdaI Nocardia dassonvillei 5' GGCGCC 3' CCGCGG 5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5' NdeI Neisseria denitrificans 5' CATATG 3' GTATAC 5' ---CA TATG--- 3' 3' ---GTAT AC--- 5' NdeII Neisseria denitrificans 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bce243I, Bsp105I, BspJI, BstMBI, CviAI, Kzo9I, NlaII, Sth368INgoAIII Neisseria gonorrhoeae FA1090 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' NgoAIV Neisseria gonorrhoeae FA1090 5' GCCGGC 3' CGGCCG 5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5' NgoMIV 1FIU, Search PDBe θ Neisseria gonorrhoeae MS11 5' GCCGGC 3' CGGCCG 5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5' NgoPII Neisseria gonorrhoeae P9-2 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' NgoPIII Neisseria gonorrhoeae P9-2 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' NheI Neisseria mucosa heidelbergensis 5' GCTAGC 3' CGATCG 5' ---G CTAGC--- 3' 3' ---CGATC G--- 5' AceII, AsuNHI, BmtI, BspOI, LlaG2I, PstNHINlaII Neisseria lactamica 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AINlaIII Neisseria lactamica 5' CATG 3' GTAC 5' ---CATG --- 3' 3' --- GTAC--- 5' NlaIV Neisseria lactamica 5' GGNNCC 3' CCNNGG 5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5' AspNI, BscBI, BmiI, BspLI, PspN4INli3877I Nostoc linckia 5' CYCGRG 3' GRGCYC 5' ---CYCGR G--- 3' 3' ---G RGCYC--- 5' Ama87I, AvaI, BcoI, BsiHKCI, Eco27kI, Eco88I, NspSAI, OfoINmeCI Neisseria meningitidis C114 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AINmeRI Neisseria meningitidis 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' NmuCI Neisseria mucosa C9-2 5' GTSAC 3' CASTG 5' --- GTSAC--- 3' 3' ---CASTG --- 5' NopI Nocardia opaca 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' NotI , 3BVQ, Search PDBe Nocardia otitidis-caviarum 5' GCGGCCGC 3' CGCCGGCG 5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5' NphI Neisseria pharyngis C245 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, RalF40I, Sau3AINruI Nocardia rubra 5' TCGCGA 3' AGCGCT 5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5' NruGI Nocardia sp. G 5' GACN5GTC 3' CTGN5CAG 5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5' AhdI, AspEI, BmeRI, BspOVI,