Nespoli is an Italian surname. Notable people with the surname include:
Gian Luigi Nespoli (1936–2007), Italian-born Cuban poet and writer Mauro Nespoli (born 1987), Italian archer Paolo Nespoli (born 1957), Italian astronaut and engineer Uriel Nespoli (1884–1973), Italian conductor
See also 12405 Nespoli, a main-belt asteroid