DGLUCY (D-glutamate cyclase) is a protein that in humans is encoded by the DGLUCY gene.
Orthologs
The human gene, DGLUCY, is highly conserved in mammals and birds.BLAST. NCBI. accessed 19 April 2010. https://blast.ncbi.nlm.nih.gov/Blast.cgi Orthologs gathered from BLAST and BLAT searches reveal that the human DGLUCY mRNA sequence is conserved with a sequence identity of 98% in chimpanzees, 88% in mice, and 81% in platypus and chicken.UCSC Genome Browser website, BLAT. accessed 10 April 2010.BLAST. NCBI. accessed 19 April 2010. The following table contains a list orthologs that were gathered from BLAST searches. Sequence alignments were performed using blastn to derive sequence identity, score, and E-values between the human c14orf159 variant 1 mRNA and its orthologs.
Genus and species Common name NCBI accession number Sequence length (bp) Sequence identity Score E-valueHomo sapiensHumanNM_0011023663164100%0Pan troglodytesChimpanzeeXM_510121297498%42810Mus musculusMouseNM_145448323188%4950Ornithorhynchus anatinusPlatypusXM_00154336.1196281%2170Gallus gallusChickenXM_421319338981500+ Human C14orf159 Orthologs-mRNA
The protein that the human gene DGLUCY encodes has been found to be highly conserved among mammals, birds, amphibians, fish, tunicates, cnidarians, and echinoderms. However, no protein orthologs have been found in nematodes, arthropods, fungi, protists, plants, bacteria, or archea. Fungi and bacteria contain the DUF1445 conserved domain which is found in human c14orf159 and its orthologs. BLAST and BLAT searches have been utilized to find orthologs to the c14orf159 protein. The following table lists protein orthologs for the human protein with sequence identity, sequence similarity, scores, and E-values derived from blastp sequence comparisons.Blastp. NCBI. https://blast.ncbi.nlm.nih.gov/Blast.cgi
Genus and species Common name NCBI accession number Sequence length-amino acids Sequence identity Sequence similarity Score E-valueHomo sapiensHumanNP_001095839.1564100%100%0Pan troglodytesChimpanzeeXP_510121.2724557/621 (89%)561/621 (90%)11090Ailuropoda melanoleucaPandaEFB15996.1585413/585 (70%)461/585 (78%)8240Rattus norvegicusRatXP_343096.2618423/618 (68%)470/618 (76%)7740Mus musculusMouseNP_663423.2617414/623 (66%)468/621 (75%)7960Equus caballusHorseXP_001916913.1581390/585 (66%)433/585 (74%)7286E-115Ornithorhynchus anatinusPlatypusXP_001514386.1653358/628 (57%)443/628 (70%)6960Gallus gallusChickenXP_421319.2617330/614 (53%)414/614 (67%)6300Xenopus tropicalisWestern clawed frogCAJ82045.1616302/611 (49%)399/611 (65%)5821E-170Danio rerioZebrafishAAI244131.1621284/607 (46%)386/607 (63%)5306E-155Branchiostoma floridaeLanceletXP_002612376.1615237/611 (38%)334/611 (54%)3976E-115Ciona intestinalisVase tunicateXP_001173256486161/501 (32%)241/501 (48%)2445E-69Strongylocentrotus purpuratusCalifornia purple sea urchinXP_782739.16319/33 (27%)15/33 (45%)3205E-87Nematostella vectensisStarlet sea anemoneXP_001637867529134/501 (26%)211/501 (42%)1201E-31+ Human C14orf159 Orthologs-protein
Post-translational modification
The protein product of the DGLUCY gene is predicted and was found to be translocated to mitochondrion.
Post-translational modifications are predicted for the protein DGLUCY. All predicted sites in human DGLUCY were compared to orthologs using multiple sequence alignments to determine likelihood of modification.Prediction of glycosylation across the human proteome and the correlation to protein function. Gupta, R. and S. Brunak. Pacific Symposium on Biocomputing, 7:310-322, 2002 <http://www.cbs.dtu.dk/services/YinOYang/>.Locating proteins in the cell using TargetP, SignalP, and related tools Olof Emanuelsson, Søren Brunak, Gunnar von Heijne, Henrik Nielsen Nature Protocols 2, 953-971 (2007) http://www.cbs.dtu.dk/services/SignalP/.Scanning the available Dictyostelium discoideum proteome for O-linked GlcNAc glycosylation sites using neural networks. R. Gupta, E. Jung, A.A. Gooley, K.L. Williams, S. Brunak and J. Hansen. Glycobiology: 9(10):1009-22, 1999 http://www.cbs.dtu.dk/services/DictyOGlyc/.Analysis and prediction of mammalian protein glycation. Morten Bo Johansen, Lars Kiemer and Søren Brunak Glycobiology, 16:844-853, 2006 http://www.cbs.dtu.dk/services/NetGlycate/. Sulfinator. Expasy tools. 2010. http://expasy.org/tools/sulfinator/.
Regulation
Estrogen receptor alpha, in the presence of estradiol, binds to the DGLUCY gene and likely regulates its expression.
References
External links
Further reading