Pantetheine kinase () is an enzyme that catalyzes the chemical reaction

The enzyme converts pantetheine to phosphopantetheine by transferring a phosphate group from the cofactor, adenosine triphosphate (ATP), which is converted to adenosine diphosphate (ADP). This is part of the biosynthetic pathway to coenzyme A.

This enzyme is a transferases, specifically one transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:pantetheine 4'-phosphotransferase. It is also called pantetheine kinase (phosphorylating).

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