Acetylputrescine deacetylase () is an enzyme characterised from Micrococcus luteus that catalyzes the hydrolysis of N-acetylputrescine to putrescine and acetic acid:

This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acetylputrescine acetylhydrolase.

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