Carboxymethylenebutenolidase (, also known as CMBL and dienelactone hydrolase) is an enzyme that catalyzes the chemical reaction
The enzyme characterised from Pseudomonas species hydrolyses cis-4-carboxymethylenebut-2-en-4-olide to maleylacetic acid. This is a step in the breakdown of 3-chlorobenzoic acid in this bacteria. A similar enzyme has been found in humans. It is also present in leafcutter ant fungal gardens.
This enzyme is a hydrolase, specifically one acting on carboxylic ester bonds. The systematic name of this enzyme class is 4-carboxymethylenebut-2-en-4-olide lactonohydrolase. Other names in use include maleylacetate enol-lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase.
Structural studies As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes , , , , , , , , , and .
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