6-acetylglucose deacetylase (EC 3.1.1.33) is an enzyme that catalyzes the reaction

The enzyme characterised from Bacillus megaterium hydrolyses 6-O-acetyl-β-D-glucose to D-glucose and acetic acid.

This enzyme is a hydrolase, specifically one acting on carboxylic ester bonds. The systematic name of this enzyme class is 6-acetyl-D-glucose acetylhydrolase. This enzyme is also called 6-O-acetylglucose deacetylase.

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