Isoflavone 3'-hydroxylase (, Formerly ) is an enzyme that catalyzes the chemical reaction
Isoflavone 3'-hydroxylase is a cytochrome P450 protein containing heme. It requires a partner cytochrome P450 reductase for functional expression. This uses nicotinamide adenine dinucleotide phosphate (NADPH). The systematic name of this enzyme class is formononetin,NADPH:oxygen oxidoreductase (3'-hydroxylating). It is also called isoflavone 3'-monooxygenase.
The enzyme isolated from chickpea is part of the biosynthetic pathway to pterocarpans which act as phytoalexins.
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