Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen.
The commonly known types are: Single diffusion in one dimension (Oudin procedure) Double diffusion in one dimension (Oakley Fulthorpe procedure) Single diffusion in two dimensions (radial immunodiffusion or Mancini method) Photograph of precipitin circles in a Petri dish during radial immunodiffusion. Double diffusion in two dimensions (Ouchterlony double immunodiffusion) Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity.
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