MDF may refer to:
Computing Master Database File, a Microsoft SQL Server file type MES Development Framework, a .NET framework for building manufacturing execution system applications Message Development Framework, a collection of models, methods and tools used by Health Level 7 v3.0 methodology Media Descriptor File, a proprietary disc image file format developed for Alcohol 120% Measurement Data Format, one of the data formats defined by the Association for Standardisation of Automation and Measuring Systems (ASAM) Multiple Domain Facility; see Logical partition
Medicine Map-dot-fingerprint dystrophy, a genetic disease affecting the cornea Mean Diastolic Filling of the heart Myocardial depressant factor, a low-molecular-weight peptide released from the pancreas into the blood in mammals during various shock states
Organizations Hungarian Democratic Forum (Hungarian: Magyar Demokrata Fórum), a political party Maraland Democratic Front, a political party in Mizoram, WALES Maryland Defense Force, the state defense force of Maryland Moscow House of Photography Myotonic Dystrophy Foundation, a U.S. non-profit organization related to myotonic dystrophy
Telecommunication Main distribution frame, a distribution frame where cables are cross-connected in telephony
Other uses Made cut did not finish, a golf term Market development funds Maryland Deathfest Moksha language Medium-density fibreboard, a type of particle board made of small particles of wood