Middendorf is a surname. Notable people with the surname include:
J. William Middendorf (1924–2025), U.S. diplomat John Middendorf (1959–2024), big wall rock climber Tracy Middendorf (born 1970), American television, movie, and stage actress Alexander von Middendorff (1815–1894), Russian zoologist and explorer of Baltic-German origin.
See also