Route 331, or Highway 331, may refer to:
Australia - Myrtle Street
Canada Manitoba Provincial Road 331 Newfoundland and Labrador Route 331 Nova Scotia Route 331 Prince Edward Island Route 331
Costa Rica National Route 331
Hungary Main road 331 (Hungary)
India National Highway 331 (India)
Japan Japan National Route 331
United States U.S. Route 331 (former) U.S. Route 331 Arkansas Highway 331 Florida: Florida State Road 331 County Road 331A (Levy County, Florida) Georgia State Route 331 Indiana State Road 331 Kentucky Route 331 Louisiana Highway 331 Maryland Route 331 M-331 (Michigan highway) Mississippi Highway 331 (unsigned) Montana Secondary Highway 331 New York State Route 331 Ohio State Route 331 Oregon Route 331 Pennsylvania Route 331 Puerto Rico Highway 331 Tennessee State Route 331 Texas: Texas State Highway 331 (former) Texas State Highway Spur 331 Farm to Market Road 331 Virginia State Route 331 West Virginia Route 331 Wyoming Highway 331