Route 301 or Highway 301 could refer to:
Canada Manitoba Provincial Road 301 Newfoundland and Labrador Route 301 Nova Scotia Route 301 Prince Edward Island Route 301 Quebec Route 301 Saskatchewan Highway 301
China China National Highway 301
Costa Rica National Route 301
Germany Bundesstraße 301
Japan Japan National Route 301
Korea, South Sangju–Yeongcheon Expressway
Philippines N301 highway (Philippines)
United Kingdom A301 road
United States U.S. Route 301 (former) U.S. Route 301 Arkansas Highway 301 Georgia State Route 301 Indiana State Road 301 Iowa Highway 301 (former) Kentucky Route 301 Louisiana Highway 301 Maryland Route 301 Minnesota State Highway 301 Mississippi Highway 301 New York: New York State Route 301 County Route 301 (Albany County, New York) County Route 301 (Erie County, New York) County Route 301 (Westchester County, New York) North Carolina Highway 301 (former) Ohio State Route 301 Pennsylvania Route 301 (former) Tennessee State Route 301 Texas: Texas State Highway 301 (former) Texas State Highway Loop 301 Farm to Market Road 301 Utah State Route 301 Virginia State Route 301
Other areas: Puerto Rico Highway 301 U.S. Virgin Islands Highway 301
Film Highway 301 (film), a 1950 film noir by Andrew L. Stone