State Road 25 (SR 25) is a state highway in the U.S. state of Florida. It is mainly signed as U.S. Highways: U.S. Highway 27 from Hialeah to Lady Lake and Belleview to Ocala U.S. Highway 301 from Belleview to near Sparr U.S. Highway 441 from Belleview to Lake City U.S. Highway 41 from High Springs to the Georgia state line near Jennings, connecting with Georgia State Route 7.

Route description U.S. Route 27; Miami through the Everglades Florida State Road 25 (SR 25) begins westward in the Edgewater neighborhood between the Blue on the Bay skyscraper and the Charter Club CondominiumsCharter Club Condominiums (Miami Condo Investments) on East 36th Street just before it turns into the eastbound on-ramp for Interstate 195. The route is unmarked and only after the intersection with U.S. Route 1 does SR 25 become the hidden state road for U.S. Route 27. One block west at the five-way intersection of Northeast Second Avenue and North Federal Highway, the road has a railroad crossing with the Florida East Coast Railway main line, which is also used by Brightline passenger trains. Four blocks after the crossing, North Miami Avenue provides access to I-195, Interstate 95, and the Airport Expressway. Due to the close proximity of I-195 US 27 and SR 25 pass under Interstate 95 with no access to the route, then intersects U.S. Route 441 (7th Avenue and hidden SR 7). By this time the road is in the Allapattah neighborhood.

State Road 25 has its first encounter with the Miami Metrorail system by passing under Allapattah (Metrorail station) just before the intersection with Florida State Road 933 (Northwest 12th Avenue). The next major intersection is Florida State Road 9 (Northwest 27th Avenue), although this is the penultimate intersection with a major highway for US 27 and SR 25 along 36th Street. US 27 and SR 25 pass under the Miami Metrorail a second time before crossing the Tri-Rail main line and leaving 36th Street under Florida State Toll Route 112 onto Okeechobee Road. From there, 36th Street becomes westbound Florida State Road 948 and the two routes run along the northwest side of the Miami Canal between Hialeah and Miami Springs. Nearly immediately after leaving Northwest 36th Street, the first major intersection along Okeechobee Road is Florida State Road 953, which includes a northbound flyover ramp from SR 953 to US 27. Other major intersections include the western terminus of FL 944 and the southern terminus of FL 823. One last intersection with West 19th Street is available before the road dips below the water level of the canal to pass under Okeechobee (Metrorail station), a Florida East Coast Railway line leading to the Hialeah Yard and the Hialeah Expressway (FL 934). Continuing along the side of this canal, US 27/FL 25 encounters the interchange with Florida State Road 826 which provides northbound and southbound access to SR 826 from northbound SR 25 via the same northbound ramp, containing a southbound U-Turn flyover. Northwest of there, the routes run along the border between Medley and Hialeah Gardens until it reaches the Homestead Extension of Florida's Turnpike at exit 35. While the canal continues to the northwest, the road turns straight north and runs along the eastern edge of the Everglades just before the intersection with Florida State Road 997, The routes cross the Miami-Dade-Broward County Line at the Snake Creek Canal. Once in Broward County, the routes enter the western edges of Pembroke Pines where it serves as the western terminus of Florida State Road 820 and Florida State Road 822 and then enters Southwest Ranches where it serves as the western terminus of Florida State Road 818, only to immediately cross over the South New River Canal where it enters Weston. Within that community, the routes bare to the north-northwest as it approaches the massive interchange with Interstate 75 at exit 23 which is also the eastern terminus of Alligator Alley and the site of the ghost town known as Andytown.

North of I-75, the North New River Canal begins and the Everglades are now found on both sides of the routes. Besides the swamplands, the surroundings include boat ramps, trail heads, canals, and power lines. These features continue as the routes cross the Broward-Palm Beach County Line, passing through the ghost town of Deem City. Deep into the Everglades, the road and the aligning canal curve from the northwest to straight north. Along this section, the route enters the barely existing community of Okeelanta, a location that tried and failed to become a boom town in the early 20th Century, but today is mostly used for sugar harvesting. The only resemblance to a major intersection here is Palm Beach County Road 827. The road continues past Okeelanta with the same sugar crops and little evidence that drivers have left the community. Just as the road enters the City of South Bay, US 27 and SR 25 crosses the first of numerous railroad crossings with the South Central Florida Express. The canal continues straight north while the road curves to the northwest and both routes join a concurrency with Florida State Road 80, which is not a hidden route. Shortly after the end of the west-to-north ramp from westbound SR 80, US 27 and SRs 25 and 80 cross another South Central Florida Express railroad line, which was originally the Lake Harbor Branch of the Florida East Coast Railway. After passing Palm Beach County Fire Rescue station Number 74, and the gas station across the street from it, SR 25 and its accompanying routes curves even more to the left, where they run along the south shore of Lake Okeechobee, protected by the Herbert Hoover Dike which carries the Lake Okeechobee Scenic Trail on top of it.Lake Okeechobee Scenic Trail (FloridaHikes) US 27 and SRs 25 and 80 continue northwest along the dike holding Lake Okeechobee from the road until it enters Lake Harbor where it curves straight west and runs over a pair of bridges over the Miami Canal. West of the John Stretch Memorial Park, the routes leave the city limits and curve back up to the northwest, following the Hoover Dike. The last site in the county is the Crooked Horse RV Park before crossing the Palm Beach-Hendry County Line

Florida Heartland through Central Florida

Continuing near the Herbert Hoover Dike, US 27, along with hidden SR 25 and non-hidden SR 80 pass the northern terminus of Hendry County Road 835 and eventually enters Clewiston, where it adopts the name "Sugarland Highway." A notable feature of this section of the road are the pair of bridges over two canals before running through Downtown Clewiston. While in the city, the road intersects local streets on a typical grid pattern to the south while streets such as Circle Drive, Crescent Drive and Arcadia Avenue loop around to the north only to return to US 27 and SRs 25 and 80 in the opposite order. After this, the routes face the intersection of North and South Lopez Street a pair of bridges over another Canal west of Lopez Street, and as the surroundings seem more less urban, the routes cross the South Central Florida Express railroad line again and leaves the city limits. For the rest of the journey US 27 and hidden SR 25 follows non-hidden SR 80 through the sugar cane fields of South Florida, interrupted only by a truck weigh station in the median. Florida State Road 80 leaves US 27/SR 25 at an interchange built in Whidden's Corner, then turns straight north, only to curve to the west again and cross the Mamie Langdale Memorial Bridge over the Caloosahatchee Canal where it enters the city limits of Moore Haven. State Road 25 as well as US 27 serves as the main road through Moore Haven, and runs along the right side of historic sites such as the Glades County Courthouse, as well as the Glades County Auditorium, and the Bronson Arena. After leaving the city limits, US 27 and SR 25 encounter the intersection with Florida State Road 78 and has a roughly four mile concurrency with that route, which heads towards Citrus Center, LaBelle, North Fort Myers and Pine Island Center. SR 25 curves to the northwest and crosses a railroad crossing with South Central Florida Express, then makes a left curve to run along the north side that railroad. On this segment, the routes pass by the CEMEX-owned Palmdale Sand Mines, and then Gatorama just before the intersection with what seems to be the northern terminus of Florida State Road 29, and suddenly curves more towards the north as it passes through the wetlands of Fisheating Creek before entering Palmdale where it passes by some driveways for the Scotts warehouse hidden behind some brush along the southbound lanes and the South Central Florida Express railroad line. Branching off to the northwest is Glades County Road 17 (Detjen's Dairy Road) the first of many county-maintained former segment of Florida State Road 17. In the meantime, SR 25 is taken from the northwest to the northeast by US 27 as the routes cross the Glades-Highlands County Line.

The first community within Highlands County for hidden SR 25 Venus which contains a single intersection with Glades County Road 731. Approximately 10 miles later the routes enter Bairs Den where the road faces a much more important intersection, specifically Florida State Road 70 Roughly four miles later, the routes encounter what may or may not be a county extension of SR 29 in the form of Highlands County Road 29 followed immediately by Highlands County Road 17 (Old State Route 8), which branches off to the southwest. These two intersections are found near Lake Placid, and after passing between Lake Pearl and Lake McCoy the routes enter the town that's partially named for that lake. Immediately after entering the town limits Highlands County Road 17 branches off to the northwest onto South Main Avenue. The routes turn straight north momentarily and then encounters the shared intersection with Highlands County Road 621 and East Interlake Boulevard (unmarked CR 17A). Westbound CR 621 secretly joins SR 25, and thus US 27. Two blocks later, the routes pass by the Lake Placid Tower. The road curves to the northwest after the intersection with South Lakeview Road, and descends towards the bridge under the South Central Florida Express railroad line, which welcomes southbound traffic to the town. CR 621 branches off to the west at Lake June Road, and also overlaps the northern terminus of the second Highlands CR 17. While US 27 and SR 25 curve more to the north a third CR 17 begins on the east side of the road.