MLO may refer to:

Organisations Macau Liaison Office, a government office Malibu Locals Only, a group of youths local to Malibu, California, US Misanthropic Luciferian Order, former name of the Satanic occult organisation Temple of the Black Light

Science and technology Mediolateral oblique, a standard view used in mammography Mildew resistance locus o, a plant gene family Mount Laguna Observatory, near San Diego, California, US Mauna Loa Observatory, an atmospheric baseline station near Hilo, Hawaii, US Multi-Link Operation, an IEEE 802.11be (Wi-Fi 7) technology Mycoplasma-like organism, the original name for a phytoplasma

Other uses Milos Island National Airport (IATA code), Greece Mortgage Loan Originator, a US licensed loan officer