Acetone 2,4-dinitrophenylhydrazone, or acetone-2,4-DNPH, is widely used for the analytical determination and detection of acetone and ketone bodies.
Chemistry It is a crystalline derivative formed from the reaction of acetone with 2,4-dinitrophenylhydrazine.
Environmental and emissions testing Acetone-DNPH serves a role in tracking carbonyl compounds within environmental air samples, industrial vehicle emissions, and mainstream cigarette smoke. Air is passed through cartridges coated with 2,4-DNPH; any ambient acetone traps on the matrix as acetone 2,4-dinitrophenylhydrazone. The complex is subsequently eluted and analyzed using High-Performance Liquid Chromatography or UV-Vis spectrophotometry.
Hazards According to the Globally Harmonized System of Classification and Labelling of Chemicals, acetone 2,4-dinitrophenylhydrazone carries a Warning signal word.
It is classified as a flammable solid and causes skin and eye irritation upon contact. Grounding and bonding of containment equipment is recommended during chemical transfers to minimize static discharge risks.
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