The 2007 Eastern Kentucky Colonels football team represented Eastern Kentucky University in the 2007 NCAA Division I FCS football season. The team was led by fifth-year head coach Danny Hope and played their home games at Roy Kidd Stadium in Richmond, Kentucky. The Colonels finished the season with a 9–3 overall record and an undefeated 8–0 record in Ohio Valley Conference games, making them conference champions. They qualified for the NCAA Division I Football Championship playoffs, where they lost to Richmond in the first round. Eastern Kentucky was ranked No. 13 in The Sports Network's postseason NCAA Division I FCS poll.

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