The following is a list of U.S. ambassadors to the World Trade Organization. The formal title is Chief of Mission for the Permanent Mission of the United States to the WTO with the rank of Ambassador during tenure of service. Historically, one of the Deputy United States Trade Representatives has served as the U.S. ambassador to the WTO.

List of U.S. Ambassadors to the WTO +AmbassadorImageAssumed officeLeft officeBooth Gardner19951997Rita Derrick HayesNovember 1997August 2001Linnet F. Deily20012005Peter F. AllgeierOctober 5, 2005August 2009Michael PunkeMarch 2010December 2016Dennis C. SheaMarch 13, 2018December 2020María PagánMarch 14, 2022January 20, 2025Joseph BarloonOctober 14, 2025present

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