The 1941 Tampa Spartans football team was an American football team that represented the University of Tampa as a member of the Southern Intercollegiate Athletic Association (SIAA) during the 1941 college football season. In their first year under head coach Flucie Stewart, the Spartans compiled an overall record of 5–4 with a mark of 2–2 in conference play. The team played home games at Phillips Field in Tampa, Florida.

Tampa was ranked at No. 219 (out of 681 teams) in the final rankings under the Litkenhous Difference by Score System for 1941.

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