Tsymbalyuk is a surname. Notable people with the surname include:
Alexander Tsymbalyuk (born 1976), Ukrainian bass opera singer Inna Tsymbalyuk (born 1985), Ukrainian actress, model and beauty pageant titleholder Kseniia Tsymbalyuk (born 1997), Russian road and track cyclist Yevhen Tsymbalyuk (born 1996), Ukrainian footballer Yuriy Tsymbalyuk, Soviet figure skater