Physalaemus orophilus is a species of frog in the family Leptodactylidae. It is endemic to Brazil.

Description The adult male frog measures about 21.1–24.8 mm in snout-vent length and the adult female 25.5–28.1 mm. The adult frog has a black lateral stripe from each eye to the inguinal region and a white one from the edge of the eye to the axilliary region.

Habitat This frog is found in primary forest and both advanced and new secondary forest. Scientists have found this frog between above sea level.

Some protected areas overlap the frog's known range: Reserva Particular do Patrimônio Natural da Serra do Caraça, APA Nova Era, and APA Pedra Gaforina.

Reproduction The tadpoles have been found on the bottoms of shallow, temporary ponds with vegetation. These ponds are associated with mountain streams.

Threats The IUCN classifies this frog as least concern of extinction. In some places, some of the frogs may be in danger from habitat loss associated with large- and small-scale agriculture, silviculture of non-native trees, and pasturage. Fires are often used for these types of land conversion. Subsistence-level wood collection may also be an issue.

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