Spyrou (Σπύρου) is a Greek surname. Notable people with the name include:
Athenagoras I (Spyrou) of Constantinople (1886–1972), Greek Orthodox Archbishop, Ecumenical Patriarch of Constantinople Artemis Spyrou, Cypriot experimental nuclear astrophysicist, professor at Michigan State University Christos Spyrou (born 1976), Greek weightlifter George Spyrou (1949–2010), Scottish businessman Loukas Spyrou (born 1973), Cypriot sprinter Panagiotis Spyrou (born 1947), Greek weightlifter Panagiotis Spyrou (weightlifter, born 2009), Greek male weightlifter Panayiotis Spyrou (born 1976) Cypriot footballer Spyros Spyrou (judoka) (born 1956), Cypriot judoka Spyros Spyrou (runner) (born 1958), Cypriot middle-distance runner
See also Spyrou Kyprianou Avenue, an important avenue in Nicosia, Cyprus formerly known as Santa Rosa Avenue