Ambati (Telugu: అంబాటి) is a Telugu surname. Notable people with the surname include:
Ambati Brahmanaiah (1939–2013), Indian politician Ambati Prudhvi Reddy (born 1996), Indian basketball player Ambati Rambabu (born 1956), Indian politician Ambati Rayudu (born 1985), Indian cricketer Balamurali Ambati (born 1977), Indian-American ophthalmologist, educator, and researcher