Do Marcolino is a Gabonese surname. Notable people with the surname include:
Alan Do Marcolino (born 2002), Gabonese football striker Arsène Do Marcolino (born 1986), Gabonese football defender Fabrice Do Marcolino (born 1983), Gabonese football striker Jonathan Do Marcolino (born 2006), Gabonese football left-back Henrick Do Marcolino (born 2006), French football midfielder