Willistonininae is a subfamily of robber flies in the family Asilidae. There are about 8 genera and more than 50 described species in Willistonininae, found in Africa, North America, and the Palearctic.

Genera These eight genera belong to the subfamily Willistonininae: Ablautus Loew, 1866 (western North America) Acnephalomyia Londt, 2010 (Afrotropical realm|Afrotropical) Ammodaimon Londt, 1985 (Afrotropical) Astiptomyia Londt, 2010 (Afrotropical) Sisyrnodytes Loew, 1856 (Palearctic) Sporadothrix Hermann, 1908 (Afrotropical) Trichoura Londt, 1994 (Afrotropical) Willistonina Back, 1909 (western North America)

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