Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A. The first two OMIP articles were published in September 2010 issue of this journal.

Purpose The goals of OMIPs are: to alleviate the development time for researchers who wish to use the same (or highly similar) panels to provide a starting point for the creation of novel OMIPs, and to provide a mechanism for attribution to the developers of the panel via citation of the publication.

List

No. No. of colors Purpose Species Cell types Paper Year Cytometry OMIP-001 16 T-cell cytokine production after in vitro stimulation and phenotyping of cytokine-producing T-cells Human PBMC 2010 BD LSR II OMIP-002 16 Specificity and phenotype of CD8+ T-cells Human PBMC, bone marrow mononuclear cells 2010 BD LSR II OMIP-003 12 Phenotyping memory B cells human PBMC, bone marrow, and tonsil mononuclear cell 2011 BD LSR II OMIP-004 15 Determine Treg levels and to provide an in depth characterization of Tregs Human PBMC, ACK-lysed blood 2012 BD LSRFortessa OMIP-005 11 T cell cytokine production and memory phenotype Rhesus macaque PBMC 2012 BD LSR II OMIP-006 9 Enumerating and phenotyping of T regulatory cells and subsets Human PBMC 2012 BD LSR II OMIP-007 14 Characterize the phenotype, maturation, and activation of NK cells Human PBMC 2012 OMIP-008 11 T cell cytokine production following in vitro stimulation Human PBMC, T cell clones/lines, tumor-resident T cell lines 2012 OMIP-009 10 phenotype and function of CD4+ and CD8+ T-cells Human PBMC 2012 OMIP-010 10 immunophenotyping of small hematopoietic cell Human Cerebrospinal fluid, bone marrow, peripheral blood 2012 OMIP-011 8 Characterization of CECs in peripheral blood Human Whole blood, RBC-lysed and washed 2012 OMIP-012 9 Human leukocyte reconstitution and phenotype Humanized mouse Whole blood, Ficoll-separated PBMC, splenocytes 2012 OMIP-013 13 T-cell differentiation Human PBMC 2012 OMIP-014 12 T-cell cytokine production and function after in vitro stimulation Human Cryopreserved PBMC 2012 OMIP-015 13 T-reg characterization and T-cell activation Human PBMC 2013 OMIP-016 10 Quality and phenotype of antigen-specific CD4 and CD8 T cells Cynomolgus macaque or Human Fresh or cryopreserved PBMCs 2013 OMIP-017 13 CD4+ helper T-cell subsets (Th1, Th2, Th9, Th17, Th17Th1, Th22 and TFH) Human PBMC 2013 OMIP-018 15 Phenotype of CD4 T cell subsets via chemokine receptor expression Human Fresh PBMC, Bone marrow mononuclear cells 2013 OMIP-019 14 γδT-cells, iNKT-cells, haematopoietic precursors Human PBMC 2013 OMIP-020 12 γδ T-cell differentiation phenotype Human PBMC 2014 OMIP-021 14 NKT cells, γδ T-cells, MAIT cells, Memory T cell subsets Human PBMC 2014 OMIP-022 14 T-cell phenotyping, memory categorization, cytokine production, and function following in vitro stimulation Human Cryopreserved PBMC (adult and infant) 2014 OMIP-023 10 In-depth phenotyping of the main leukocyte subtypes in one run Human Fresh EDTA-treated peripheral blood, RBC-lysed and Fresh EDTA-treated Cord-blood 2014 OMIP-024 18 Characterize the phenotype of PBMC leukocyte subsets Human Cryopreserved PBMC from adults and infants 2014 OMIP-025 16 Characterization of antigen-specific T cells, TFH-like cells and NK cells Human Cryopreserved PBMC 2014 OMIP-026 14 Phenotype of B and plasma cells Rhesus macaque, African green monkey Fresh whole blood, PBMCs, mononuclear cells of bone marrow, lymph node, spleen, tonsil 2015 OMIP-027 OMIP-028 OMIP-029 OMIP-030 OMIP-031 OMIP-032 OMIP-033 OMIP-034 OMIP-035 OMIP-036 OMIP-037 OMIP-038 OMIP-039 OMIP-040 OMIP-041 OMIP-042 OMIP-043 OMIP-044 OMIP-045 OMIP-046 OMIP-047 OMIP-048 OMIP-049 19 Subset analysis of hematopoiesis and hematopoietic disease Human Any source containing human myeloid cells 2018 BD LSR II OMIP-050 28 OMIP-051 OMIP-052 OMIP-053 OMIP-054 OMIP-055 OMIP-056 OMIP-057 OMIP-058 OMIP-059 OMIP-060 OMIP-061 OMIP-062 14 Myeloid and innate/adaptive lymphoid comprehensive immunophenotyping Human Whole peripheral blood, cord blood, PBMCs, nasal aspirate, and tracheal aspirate samples 2019 BD LSRFortessa OMIP-063 OMIP-064 OMIP-065 OMIP-066 OMIP-067 OMIP-068 OMIP-069 OMIP-070 OMIP-071 OMIP-072 OMIP-073 OMIP-074 OMIP-075 OMIP-076 OMIP-077 OMIP-078 OMIP-079 OMIP-080 OMIP-081 OMIP-082 25 OMIP-083 18 OMIP-084 28 OMIP-085 8 OMIP-086 22 OMIP-087 OMIP-088 OMIP-089 8 OMIP-090 18 OMIP-091 27 OMIP-092 6 OMIP-093 41 OMIP-094 24 OMIP-095 OMIP-096 OMIP-097 OMIP-098 OMIP-099 OMIP-100 OMIP-101 27 Broad immunophenotyping of leukocytes including myeloid (neutrophils, monocytes, mDCs) and lymphoid cell lineages (conventional T cells, DURT cells, γδT cells, B cells, NK cells, ILCs) Human Fresh or fixed, cryopreserved whole blood 2024 BD FACSymphony A5

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