The 2000 Bethune–Cookman Wildcats football team represented Bethune–Cookman College (now known as Bethune–Cookman University) as a member of the Mid-Eastern Athletic Conference (MEAC) during the 2000 NCAA Division I-AA football season. Led by fourth-year head coach Alvin Wyatt, the Wildcats compiled an overall record of 9–2, with a mark of 6–2 in conference play, and finished tied for second in the MEAC.

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