The women's 400 metres event at the 2025 Summer World University Games was held in Bochum, Germany, at Lohrheidestadion on 21, 22 and 23 July.

Records Prior to the competition, the records were as follows: Record Athlete (nation) Location Date Games record 49.88 Palma de Mallorca, Spain 12 July 1999

Results Heats First 3 in each heat (Q) and the next 6 fastest (q) qualified for the semi-finals.

Heat 1 Place Athlete Nation Time Notes 1 52.68 2 Maryana Shostak 52.76 , 3 Alessandra Bonora 53.39 4 53.96 5 54.64 6 Itzel Cervantes 56.54 7 Evelīna Krakope 60.74 8 Malaika Shahzadi 64.04

Heat 2 Place Athlete Nation Time Notes 1 Jana Lakner 52.26 2 Kinga Gacka 53.12 3 Precious Molepo 53.48 4 53.49 5 Paige Willems 54.27 6 56.36 7 Martha Spiteri 56.60

Heat 3 Place Athlete Nation Time Notes 1 Aleksandra Formella 52.66 2 Favour Okpali 52.96 3 53.81 4 54.02 5 55.04 6 Isabelle Skelton 55.65

Heat 4 Place Athlete Nation Time Notes 1 Veronika Drljacic 52.80 2 53.35 3 53.75 4 54.35 5 Jonbibi Hukmova 55.21 6 Luciana Quinto 57.03 7 Dorothy Abeja 58.87

Heat 5 Place Athlete Nation Time Notes 1 Charlee Crawford 53.52 2 Barbora Malíková 53.62 3 54.13 4 55.90 5 Iris Morales 56.39 6 Chioma Christabel Offor 59.81 7 Christine Kabwe 63.87

Heat 6 Place Athlete Nation Time Notes 1 Marlie Viljoen 53.48 2 Annalies Kalma 53.89 3 54.42 4 54.60 5 55.55 6 Pamela Nicol Barreto 56.64 7 Kim Thanh Nguyen Thi 58.28

Semi-finals First 2 in each heat (Q) and the next 2 fastest (q) qualified for the final.

Heat 1 Place Athlete Nation Time Notes 1 Veronika Drljacic 52.13 2 52.44 3 Kinga Gacka 52.86 = 4 52.95 5 53.15 6 Precious Molepo 53.30 7 53.72 8 Paige Willems 54.82

Heat 2 Place Athlete Nation Time Notes 1 Barbora Malíková 52.46 2 Aleksandra Formella 52.62 3 Favour Okpali 52.84 4 Marlie Viljoen 52.99 5 53.68 6 54.26 7 54.49 8 55.33

Heat 3 Place Athlete Nation Time Notes 1 Jana Lakner 52.37 2 Alessandra Bonora 52.63 3 Charlee Crawford 52.82 4 Maryana Shostak 52.95 5 Annalies Kalma 53.63 6 54.11 7 54.46 8 54.48

Final Place Athlete Nation Time Notes Barbora Malíková 51.52 Veronika Drljacic 51.66 Alessandra Bonora 52.41 4 Aleksandra Formella 52.56 5 Jana Lakner 52.57 6 52.83 7 Charlee Crawford 52.85 8 Favour Okpali 53.87

References