Middlemore is a surname, and may refer to:
Amphilis Throckmorton Middlemore (1891–1931), daughter of Sir John Middlemore George Middlemore (died 1850), British Army officer and politician Henry Middlemore (died 1592), English courtier Humphrey Middlemore (died 1535), English Catholic priest and Carthusian hermit John Middlemore (1844–1924), baronet, politician, brother of Thomas Middlemore Maria Trinidad Howard Sturgis Middlemore (1846–1890), author, wife of Samuel George Chetwynd Middlemore] Mary Middlemore (died 1618), English courtier Samuel George Chetwynd Middlemore (1848–1890), English translator and journalist Thomas Middlemore (1842–1923), English mountaineer and head of Middlemores Saddles