The 2001 Samford Bulldogs football team represented Samford University as an independent during the 2000 NCAA Division I-AA football season, and the Bulldogs compiled an overall record of 4–7. The team played home games at Seibert Stadium in Homewood, Alabama. Eighth-year head coach Pete Hurt led Samford to a 1–4 record before he was fired mid-season. Bill Gray served as head coach for the remainder of the season and went 4–1.

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