The 1995 Eastern Kentucky Colonels football team represented Eastern Kentucky University as a member of the Ohio Valley Conference (OVC) during the 1995 NCAA Division I-AA football season. Led by 31st-year head coach Roy Kidd, the Colonels compiled an overall record of 9–3, with a mark of 7–1 in conference play, and finished second in the OVC. Eastern Kentucky advanced to the NCAA Division I-AA playoffs and were defeated by Montana in the first round.

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