Anca Todoni was the defending champion and successfully retained the singles title at the 2025 Open delle Puglie, defeating Anna Bondár in the final, 6–7(4–7), 6–4, 6–4.
Seeds
Draw
Finals
Top half
Bottom half
Qualifying Seeds
Qualifiers
Qualifying draw
First qualifier
Second qualifier
Third qualifier
Fourth qualifier
References
External links Main draw Qualifying draw