Barnes Island (also called Barn IslandSailing Directions for Nova Scotia, 1952, US Navy Hydrographic Office) is an undeveloped 33-acre island in the West Isles Parish of Charlotte County, New Brunswick, Canada, where the Bay of Fundy enters Passamaquoddy Bay.

Covered in mixed soft and hardwood, and raspberries, the island is privately owned by the New Brunswick Land Trust with an easement for the Nature Trust of New Brunswick. It is under the protection of the Nature Conservancy of Canada.

It was closed to public camping in 1997. In 2016, 2022 and 2025 volunteers with the Trust helped clean the island's shores.

In 1974, a stranded white-sided dolphin was found on the island. In the late 1970s it was noted to no longer host previous nesting bald eagles, although it had kittiwake and osprey populations.

It was one of four islands studied in 1997 for the impact of sea kayaking on the Bay of Fundy environment. In Summer 2012, the island was one of four studied as a rockweed habitat.

Alonzo "Lon" Greenlaw was born on Barnes Island to Warren Greenlaw prior to the latter's move to Conley's Point in Leonardville.Welch, H. Wesley. "Welcome to Deer Island: Deer Island History", 1967. In reference collection St. Croix Library. Pg54 In 1884, 15-year old William Thornton died on Barnes Island after a "life marked by suffering". In 1911, US Civil War veteran Levi Call sent his sons to Deer Island for a doctor complaining of feeling ill, then retired to his bedroom on Barnes Island and killed himself with a shotgun blast to the face.The Semi-weekly telegraph and The News : Vol. L, No. 38 (February 11, 1911)The Granite town greetings : Vol. 6, No. 31 (February 10, 1911)The Daily Gleaner : November 23, 1910-April 10, 1911

In December 1985, a study by Parks Canada assessed the island's value as $24,200.Parks Canada, "West Isles Feasibility Study ... a National Marine Park in the West Isles", December 1985

In 2016, the island was one of the focuses of the Great Fundy Cleanup.

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