The 1994 Duquesne Dukes football team represented Duquesne University as a member of the Metro Atlantic Athletic Conference (MAAC) during the 1994 NCAA Division I-AA football season. Led by second-year head coach Greg Gattuso, the team compiled an overall record of 6–4, with a mark of 4–3 in conference play, and finished tied for third in the MAAC.

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