The 1941 Tuskegee Golden Tigers football team represented the Tuskegee Institute—now known as Tuskegee University—as a member of the Southern Intercollegiate Athletic Conference (SIAC) during the 1941 college football season. Led by 18th-year head coach Cleve Abbott, they Golden Tigers compiled an overall record of 8–2 with mark of 6–1 in conference play. Tuskegee was invited to the Orange Blossom Classic, where the Golden Tigers lost to Florida A&M. The team played home games at the Alumni Bowl in Tuskegee, Alabama.
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