Ladies' Day may refer to:
Ladies' Day (baseball), a promotional event in Major League Baseball during the 20th century
Ladies' Day (film), a 1943 film by Leslie Goodwins
Related Ascot Gold Cup, held on the third day of the Royal Ascot races which is unofficially called "Ladies' Day" Ladies Day Vase, a Thoroughbred horse race for mares held at Caulfield Racecourse in Melbourne, Australia
See also Ladies' Night (disambiguation)