The 2000 Arkansas–Pine Bluff Golden Lions football team represented the University of Arkansas at Pine Bluff as a member of the Southwestern Athletic Conference (SWAC) during the 2000 NCAA Division I-AA football season. Led by eighth-year head coach Lee Hardman, the Golden Lions compiled an overall record of 7–4, with a mark of 4–3 in conference play, and finished tied for third in the SWAC West Division.
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