BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi. It was first described by in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981. The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.
Typical composition
BAF agar typically contains:
30.0 g/L glucose 2.0 g/L peptone 0.2 g/L yeast extract 0.5 g/L KH2PO4 0.5 g/L MgSO4.7 H2O 10.0 mg/L FeCl3.6 H2O 1.0 mg/L ZnSO4.7 H2O 5.0 mg/L MnSO4 100.0 mg/L CaCl2.2 H2O 50.0 μg/L thiamine HCl 1.0 μg/L biotin 100.0 μg/L folic acid 50.0 μg/L inositol 15 g/L agar
References